covaris e220evolution (Covaris)

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Covaris covaris e220evolution
Covaris E220evolution, supplied by Covaris, used in various techniques. Bioz Stars score: 96/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/covaris+e220evolution/E220evolution+Focused-ultrasonicator/10__1016_slash_j__soi__2026__100247-55-38-38
Average 96 stars, based on 171 article reviews

covaris e220evolution - by Bioz Stars, 2026-10

96/100 stars

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Article Title: Antagonism among DUX family members evolved from an ancestral toxic single homeodomain protein.
Article Snippet: The other set (Figure 4) was prepared and sequenced (2 3 150 paired-end) on an Illumina HiSeq at Genewiz (NJ, USA).

Isolation:

Article Title: Antagonism among DUX family members evolved from an ancestral toxic single homeodomain protein
Article Snippet: 24 h after later, gene expression was induced by addition of Doxycycline (Sigma, D9891-10G) to a final concentration of 100 ng/ml for 24 h. Next, chromatin-protein complexes were cross-linked by addition of methanol-free formaldehyde (ThermoFisher, 28906) at a final concentration of 1 % for 10 min at RT. .. Cells were then washed three times in PBS and chromatin was isolated and sheared to 200-700 bp using the truChIP Chromatin Shearing Kit (Covaris, 520154) and a Covaris E220evolution focused-ultrasonicator following the manufacturer’s recommendations. .. Subsequently, 30 μg of chromatin was diluted 1:4 with RIPA-buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0, 140 mM NaCl, 1 % Triton X-100, 0.1 % sodium deoxycholate) and incubated with 4 μg anti-V5-antibody (Abcam, ab9116) at 4°C overnight.

Purification:

Article Title: Targeting the EGFR pathway in chemotherapy-resistant triple-negative breast cancer - a phase II study
Article Snippet: .. RNA was extracted (Norgen Total RNA Purification Kit; Cat. 37500), treated with DNase I to eliminate genomic DNA residues, and purified (AMPure XP beads; Beckman Coulter Life Sciences). cDNA was prepared (Ovation RNASeq System V2; NuGEN) and up to 200 ng of cDNA was sheared (Covaris E220evolution Focused-ultrasonicator). .. Library preparation and hybridization were performed (Sciclone G3 NGSx Workstation; PerkinElmer, Inc.) using Agilent SureSelectXT Low Input Reagent Kit with indexes 1 to 96 and Agilent SureSelect Human All Exon v.4 probes.

RNA sequencing:

Lysis:

Article Title: Incidence and Timing of Recurrence After Adjuvant Palbociclib in Completely Resected Retroperitoneal Liposarcoma
Article Snippet: RNA was extracted from FFPE tissue using the Covaris truXTRAC FFPE RNA Plus Kit (SKU 520271), following the manufacturer’s instructions with minor modifications. .. Briefly, two 10 μm scrolls (maximum tissue area 10 × 10 mm area per slide) were transferred into microTUBE-500 Screw Cap tubes containing 440 μl RNA Lysis Buffer and 44 μl Proteinase K. Tubes were processed on the Covaris E220evolution Focused-ultrasonicator using the “Acoustic Paraffin Emulsification” setting (PIP 200 W, DF 10%, CPB 200, 300 s at 20 °C bath temp, water level 6). ..

Emulsification:

Sonication:

Article Title: Functional dissection of H3K4 methyltransferases reveals distinct catalytic and non-catalytic roles in C. elegans development
Article Snippet: .. Sonication was performed using the Covaris E220evolution with the following conditions: PIP: 175, DF: 10%, CPB: 200 10-15 min. .. The following antibodies were used: anti-H3K4me3 (abcam, ab8580; 1:500) and anti H3K4me1 (abcam, ab8895; 1:500).

Article Title: Inter and transgenerational impact of H3K4 methylation in neuronal homeostasis.
Article Snippet: .. The samples were sonicated using a Covaris E220evolution to 100–500 bp. .. DNA was purified using QIAGEN minElute PCR purification columns and DNA libraries were generated using the NEB Next Ultra DNA library preparation kit for Illumina (Cat no. E7370).

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